Page 47 - MDA MIDEC 2024 Programme Book

ORAL PRESENTATION ABSTRACTS

P004 Name of Presenter: Nicholas Sim Choo Wee, University Malaya
Title of Presentation: Supragingival Bacterial Microbiota Around Dental Implants & The
Adjacent Tooth In Patients With A History Of Periodontitis

Abstract
Sim NCW 1, Anis Rageh AM 2, Eshamsul S 1, Norul Husna MH 1, Syarida Hasnur S 1,*
1 Department of Restorative Dentistry, Faculty of Dentistry, Universiti Malaya, Kuala Lumpur, Malaysia
2 Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia
Objectives: The aim of this study was to investigate a causal link between the oral microbiota dysbiosis and
the risk of peri-implantitis in patients with a history of periodontitis.
Materials and Methods: Subjects with a history of periodontitis (Test) and periodontally healthy subjects
(Control) were recruited to receive either of two types of dental implants with different surface characteristics:
sandblasted, large grit and acid-etched (SLA) or precision dimension laser-treated (PDL). Periodontal clinical
measurements were recorded at baseline (V1), 3 months after implant placement (V4), at zirconia crown
placement (V6) and 3 months after zirconia crown placement (V8). Supragingival bacterial microbiota was
studied using Illumina MiSeq sequencing.
Results: Supragingival microbial community on SLA implants in the test group significantly differed to the
control group at V8 (p < 0.05). After three months of zirconia crown restoration (V6 to V8), a longitudinal shift
of the supragingival bacterial microbiome displaying microbial dysbiosis occurred on the SLA implants (p
< 0.05) and the adjacent teeth (p < 0.05) among test patients. Supragingival bacterial microbiome on PDL
implants and the respective adjacent tooth did not differ significantly between test and control groups
from V6 to V8 (p > 0.05). Co-occurrence network in the test group of SLA implants at V8 and the adjacent
tooth displayed increased disease-associated bacteria and reduced health-associated bacteria. Health-
associated bacteria were dominant in the control group of SLA implants at V8.
Conclusion: The surface characteristics and prosthetic component of dental implants could be considered
as important risk factors for the onset of microbial dysbiosis in patients with a history of periodontitis.
Dysbiosis of supragingival microbiome in patients with a history of periodontitis may predispose dental
implants to peri-implant mucositis or peri-implantitis, thus, a strict supportive periodontal care plan is
imperative for these patients to prevent early onset of biological complications around dental implants.

Key words: History of Periodontitis, Implant, Adjacent tooth, Supragingival microbiota, 16s rRNA gene
sequencing

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